Journal: International Journal of General Medicine

Article Title: A Five-Gene PANoptosis Signature Correlates with Immune Infiltration and Secondary Brain Injury in Intracerebral Hemorrhage

doi: 10.2147/IJGM.S581629

Figure Lengend Snippet: Correlation between the five signature genes . ( A ) Chord diagram of correlation. ( B ) Heat map of correlation. ( D–M ) Scatter plot of correlation among AKR1C2, SLC2A14, FTL, TNFRSF12A, and SLC2A3. * represents p<0.05; **represents p<0.01; ***represents p<0.01.

Article Snippet: After blocking with 5% BSA, the membranes were incubated overnight at 4°C with the following primary antibodies (all at 1:1000 dilution): AKR1C2 (#13035S, Cell Signaling Technology), FTL (#ab69090, Abcam), SLC2A14 (#PK35494-S, Abmart), SLC2A3 (#20403-1-AP, Proteintech), TNFRSF12A (# AWA10766 , Abiowell), and GAPDH (#ab181602, ProteinTech).

Techniques:

Journal: International Journal of General Medicine

Article Title: A Five-Gene PANoptosis Signature Correlates with Immune Infiltration and Secondary Brain Injury in Intracerebral Hemorrhage

doi: 10.2147/IJGM.S581629

Figure Lengend Snippet: Experimental verification of protein expression in the clinical sample. ( A ) The expression levels of AKR1C2, SLC2A14, FTL, TNFRSF12A, and SLC2A3 were detected by Western blot. ( B–F ) Protein quantification of the five signature genes. * represents p<0.05; ns represents no significance.

Article Snippet: After blocking with 5% BSA, the membranes were incubated overnight at 4°C with the following primary antibodies (all at 1:1000 dilution): AKR1C2 (#13035S, Cell Signaling Technology), FTL (#ab69090, Abcam), SLC2A14 (#PK35494-S, Abmart), SLC2A3 (#20403-1-AP, Proteintech), TNFRSF12A (# AWA10766 , Abiowell), and GAPDH (#ab181602, ProteinTech).

Techniques: Expressing, Western Blot

Journal: International Journal of General Medicine

Article Title: A Five-Gene PANoptosis Signature Correlates with Immune Infiltration and Secondary Brain Injury in Intracerebral Hemorrhage

doi: 10.2147/IJGM.S581629

Figure Lengend Snippet: Correlation between the five signature genes . ( A ) Chord diagram of correlation. ( B ) Heat map of correlation. ( D–M ) Scatter plot of correlation among AKR1C2, SLC2A14, FTL, TNFRSF12A, and SLC2A3. * represents p<0.05; **represents p<0.01; ***represents p<0.01.

Article Snippet: After blocking with 5% BSA, the membranes were incubated overnight at 4°C with the following primary antibodies (all at 1:1000 dilution): AKR1C2 (#13035S, Cell Signaling Technology), FTL (#ab69090, Abcam), SLC2A14 (#PK35494-S, Abmart), SLC2A3 (#20403-1-AP, Proteintech), TNFRSF12A (# AWA10766 , Abiowell), and GAPDH (#ab181602, ProteinTech).

Techniques:

Journal: International Journal of General Medicine

Article Title: A Five-Gene PANoptosis Signature Correlates with Immune Infiltration and Secondary Brain Injury in Intracerebral Hemorrhage

doi: 10.2147/IJGM.S581629

Figure Lengend Snippet: Experimental verification of protein expression in the clinical sample. ( A ) The expression levels of AKR1C2, SLC2A14, FTL, TNFRSF12A, and SLC2A3 were detected by Western blot. ( B–F ) Protein quantification of the five signature genes. * represents p<0.05; ns represents no significance.

Article Snippet: After blocking with 5% BSA, the membranes were incubated overnight at 4°C with the following primary antibodies (all at 1:1000 dilution): AKR1C2 (#13035S, Cell Signaling Technology), FTL (#ab69090, Abcam), SLC2A14 (#PK35494-S, Abmart), SLC2A3 (#20403-1-AP, Proteintech), TNFRSF12A (# AWA10766 , Abiowell), and GAPDH (#ab181602, ProteinTech).

Techniques: Expressing, Western Blot

Journal: bioRxiv

Article Title: Endocrine-metabolic decoupling drives stress vulnerability in dystrophin deficiency

doi: 10.64898/2026.02.22.707291

Figure Lengend Snippet: a , Area under the curve (AUC) for blood glucose concentrations following a 5-h fast and intraperitoneal injection of insulin (0.5 IU kg⁻¹; insulin tolerance test (ITT)) measured at rest (non-stressed) or during a 2-h tube-restraint (stressed). b , Relative frequency distribution of pancreatic islet area from isolated islets. c , Mean pancreatic islet area. d-f , Representative immunoblot (d) and quantification of glucose transporter 3 (GLUT3) protein abundance in triceps (e) and brain (f) extracts, normalised to total protein and expressed relative to WT. g , [¹⁸F] fluorodeoxyglucose (¹⁸F-FDG) standardised uptake values (SUV) in templated brain regions from mice treated with exogeneous insulin prior to stress or non-stress conditions. Data were analysed using repeated-measures ANOVA or linear mixed-effects models with Bonferroni post-hoc adjustments, or One-way ANOVA or Kruskal-Wallis with Dunn’s post-hoc testing. n =6-8 per group. Data are mean ± SEM. * P < 0.05.

Article Snippet: After blocking in 5% BSA or skim milk in TBST for 1 h, membranes were incubated overnight at 4°C with primary antibodies for GLUT3 (ProteinTech, 1:500) or GLUT4 (Thermo Fisher, 1:500).

Techniques: Injection, Isolation, Western Blot, Quantitative Proteomics